Annexin A2 Monoclonal antibody, PBS Only

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  WB analysis using 60051-1-Ig (same clone as 60051-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60051-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 60051-1-Ig (same clone as 60051-1-PBS)

  WB result of Annexin A2 antibody (60051-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-Annexin A2 transfected THP-1 cells. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60051-1-Ig (same clone as 60051-1-PBS)

  WB result of Annexin A2 antibody (60051-1-Ig, 1:20,000) with si-Control and si-Annexin A2 transfected HEK293 cells. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)

  IP result of anti-Annexin A2 (IP:60051-1-Ig, 4ug; Detection:60051-1-Ig 1:1500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60051-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60051-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60051-1-Ig (ANXA2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60051-1-Ig (ANXA2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  IF Staining of T-47D using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 60051-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Annexin A2 antibody (60051-1-Ig, Clone: 3H1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunofluorescent analysis of Hela cells, using ANXA2 antibody 66051-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using ANXA2 antibody 60051-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 60051-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 60051-1-PBS

Clone No.3H1B11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ANXA2, 3H1B11, Annexin 2, Annexin II, Annexin-2

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Product Information

60051-1-PBS targets Annexin A2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1777

  Product name: Recombinant human Annexin A2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-339 aa of BC016774

  Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

• Full Name: annexin A2
• Calculated Molecular Weight: 339 aa, 39 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC016774
• Gene Symbol: Annexin A2
• Gene ID (NCBI): 302
• ENSEMBL Gene ID: ENSG00000182718
• RRID: AB_2057309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07355
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.