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- KD/KO Validated
Annexin V Polyclonal antibody
Annexin V Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 11060-1-AP
|Positive WB detected in||HeLa cells, A431 cells, HEK-293 cells, HepG2 cells, human heart tissue, human lung tissue, human placenta tissue, human skeletal muscle tissue, NIH/3T3 cells, pig heart tissue, pig liver tissue|
|Positive IP detected in||HeLa cells, mouse liver tissue|
|Positive IHC detected in||human spleen tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11060-1-AP targets Annexin V in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, pig samples.
|Tested Reactivity||human, mouse, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Annexin V fusion protein Ag1538|
|Full Name||annexin A5|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC018671|
|Gene ID (NCBI)||308|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.
|Product Specific Protocols|
|WB protocol for Annexin V antibody 11060-1-AP||Download protocol|
|IHC protocol for Annexin V antibody 11060-1-AP||Download protocol|
|IF protocol for Annexin V antibody 11060-1-AP||Download protocol|
|IP protocol for Annexin V antibody 11060-1-AP||Download protocol|
|FC protocol for Annexin V antibody 11060-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Vasc Interv Radiol
Usefulness of material recovered from distal embolic protection devices after carotid angioplasty for proteomic studies.
Proteomic and tissue array profiling identifies elevated hypoxia-regulated proteins in pancreatic ductal adenocarcinoma.
CTL-Derived Granzyme B Participates in Hippocampal Neuronal Apoptosis Induced by Cardiac Arrest and Resuscitation in Rats.
Calcium relieves fluoride-induced bone damage through the PI3K/AKT pathway.
J Proteome Res
Enrichment of Neurodegenerative Microglia Signature in Brain-Derived Extracellular Vesicles Isolated from Alzheimer's Disease Mouse Models.
Cell Death Dis
Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways.