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AP1M2 Polyclonal antibody, PBS Only (10618-1-PBS)

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WB analysis of Hacat cells using 10618-1-AP (same clone as 10618-1-PBS)
WB result of AP1M2 antibody (10618-1-AP;1:500) with siRNA cell lysate. This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.
WB analysis of mouse colon using 10618-1-AP (same clone as 10618-1-PBS)
mouse colon tissue were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.
WB analysis of BxPC-3 using 10618-1-AP (same clone as 10618-1-PBS)
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 10618-1-AP (same clone as 10618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 10618-1-AP (same clone as 10618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.
IHC staining of human bowen disease using 10618-1-AP (same clone as 10618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.
IHC staining of human bowen disease using 10618-1-AP (same clone as 10618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 10618-1-AP (same clone as 10618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 10618-1-AP (same clone as 10618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

AP1M2 Polyclonal antibody, PBS Only

Name: AP1M2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 10618-1-PBS

AP1M2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 10618-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

10618-1-PBS targets AP1M2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0961 Product name: Recombinant human AP1M2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-320 aa of BC005021 Sequence: MSASAVFILDVKGKPLISRNYKGDVAMSKIEHFMPLLVQREEEGALAPLLSHGQVHFLWIKHSNLYLVATTSKNANASLVYSFLYKTIEVFCEYFKELEEESIRDNFVIVYELLDELMDFGFPQTTDSKILQEYITQQSNKLETGKSRVPPTVTNAVSWRSEGIKYKKNEVFIDVIESVNLLVNANGSVLLSEIVGTIKLKVFLSGMPELRLGLNDRVLFELTGLSGSKNKSVELEDVKFHQCVRLSRFDNDRTISFIPPDGDFELMSYRLSTQVKPLIWIESVIEKFSHSRVEIMVKAKGQFKKQSVANGVEISVPVPS Predict reactive species
Full Name	adaptor-related protein complex 1, mu 2 subunit
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	50-55 kDa
GenBank Accession Number	BC005021
Gene Symbol	AP1M2
Gene ID (NCBI)	10053
RRID	AB_2242895
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y6Q5
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

AP1M2 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.