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AP2S1 Polyclonal antibody

AP2S1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15634-1-AP

Synonyms

AP 2 complex subunit sigma, AP17, AP17 DELTA, AP2S1, CLAPS2, HA2 17 kDa subunit, Sigma2 adaptin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AP2S1 Polyclonal antibody (15634-1-AP)

    WB analysis using 15634-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15634-1-AP (AP2S1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15634-1-AP targets AP2S1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP2S1 fusion protein Ag8095
Full Name adaptor-related protein complex 2, sigma 1 subunit
Calculated Molecular Weight 142 aa, 17 kDa
Observed Molecular Weight15-17 kDa
GenBank Accession NumberBC006337
Gene Symbol AP2S1
Gene ID (NCBI) 1175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP2S1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1), and a small adaptin (sigma-type subunit AP2S1). It works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis. Missense mutations of AP2S1 affect Arg15 and lead to familial hypocalciuric hypercalcemia type 3 (FHH3), an extracellular calcium homeostasis disorder affecting the parathyroids, kidneys and bone (PMID: 23222959).

Protocols

Product Specific Protocols
WB protocol for AP2S1 antibody 15634-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols