AP3B1 Polyclonal antibody, PBS Only

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• AP3B1 Polyclonal antibody, PBS Only (13384-1-PBS)

• 

  WB analysis using 13384-1-AP (same clone as 13384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13384-1-AP (AP3B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 13384-1-AP (same clone as 13384-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13384-1-AP (AP3B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

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  IP experiment of COLO 320 using 13384-1-AP (same clone as 13384-1-PBS)

  IP result of anti-AP3B1 (IP:13384-1-AP, 3ug; Detection:13384-1-AP 1:500) with COLO 320 cells lysate 2500ug. This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 13384-1-AP (same clone as 13384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13384-1-AP (AP3B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 13384-1-AP (same clone as 13384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13384-1-AP (AP3B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

AP3B1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 13384-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

13384-1-PBS targets AP3B1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4225

  Product name: Recombinant human AP3B1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 763-1094 aa of BC038444

  Sequence: KTSDSSNDESSSIEDSSSDSESESEPESESESRRVTKEKEKKTKQDRTPLTKDVSLLDLDDFNPVSTPVALPTPALSPSLMADLEGLHLSTSSSVISVSTPAFVPTKTHVLLHRMSGKGLAAHYFFPRQPCIFGDKMVSIQITLNNTTDRKIENIHIGEKKLPIGMKMHVFNPIDSLEPEGSITVSMGIDFCDSTQTASFQLCTKDDCFNVNIQPPVGELLLPVAMSEKDFKKEQGVLTGMNETSAVIIAAPQNFTPSVIFQKVVNVANVGAVPSGQDNIHRFAAKTVHSGSLMLVTVELKEGSTAQLIINTEKTVIGSVLLRELKPVLSQG

• Full Name: adaptor-related protein complex 3, beta 1 subunit
• Calculated Molecular Weight: 1094 aa, 121 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC038444
• Gene Symbol: AP3B1
• Gene ID (NCBI): 8546
• RRID: AB_2056499
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00203
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AP3B1 is the 140-kDa β3A subunit of the adaptor-related protein complex-3 (AP-3), a ubiquitous heterotetrameric complex that is localized to the trans-Golgi network and endosomes and is involved in protein trafficking to lysosomes or specialized endosomal-lysosomal organelles (PMID: 9182526; 9545220). This complex is composed of two larger subunits (δ and β3A or β3B), a medium subunit (μ3A or μ3B), and a small subunit (σ3A or σ3B). The absence of the β3A subunit (AP3B1) results in the loss of stability of AP3 and leads to degradation of μ3A, to which β3A is directly bound, while the other subunits are variably affected (PMID: 16507770). AP3B1 contains three main domains: the N-terminal head domain, the hinge, and the C-terminal ear domain. It has been reported as a target of IP(7)-mediated pyrophosphorylation (PMID: 19934039). Defects in AP3B1 are the cause of Hermansky-Pudlak syndrome type 2 (HPS2) (PMID: 10024875; 16507770).