APLP1 Recombinant monoclonal antibody

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• APLP1 Recombinant monoclonal antibody (87113-1-RR)

APLP1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 87113-1-RR

Clone No.252210C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

A4, Amyloid beta, Amyloid beta precursor like protein 1, Amyloid like protein 1, Amyloid-like protein 1

• 

  WB analysis of fetal human brain using 87113-1-RR

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 87113-1-RR (APLP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 87113-1-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 87113-1-RR (APLP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 87113-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 87113-1-RR (APLP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 87113-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 87113-1-RR (APLP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 87113-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using APLP1 antibody (87113-1-RR, Clone: 252210C4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 87113-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87113-1-RR against Human APLP1 were performed. The affinity constant is below 1 pM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87113-1-RR targets APLP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5634

  Product name: Recombinant Human APLP1 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 39-580 aa of NM_005166.4

  Sequence: SLAGGSPGAAEAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSRE

• Full Name: amyloid beta (A4) precursor-like protein 1
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: NM_005166.4
• Gene Symbol: APLP1
• Gene ID (NCBI): 333
• RRID: AB_3745348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P51693-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APLP1 (Amyloid Precursor-Like Protein 1) is a member of the amyloid precursor protein (APP) family, which also includes APP and APLP2. It is a type I transmembrane glycoprotein that is highly and predominantly expressed in the central nervous system, especially at the postsynaptic density of neurons. Functionally, APLP1 plays important roles in synaptic adhesion, synaptogenesis, and neurite outgrowth. It can form homophilic trans-interactions between neighboring cells, contributing to cell-cell adhesion and the formation of synaptic structures. Its proteolytic processing by α-, β-, and γ-secretases releases intracellular fragments that may participate in transcriptional regulation and signal transduction, potentially influencing gene expression and neuronal survival.