APOF Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• APOF Polyclonal antibody, PBS Only (16608-1-PBS)

• 

  WB analysis of human plasma using 16608-1-AP (same clone as 16608-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 16608-1-AP (APOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16608-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16608-1-AP (same clone as 16608-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16608-1-AP (APOF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16608-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16608-1-AP (same clone as 16608-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16608-1-AP (APOF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16608-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16608-1-AP (same clone as 16608-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16608-1-AP (APOF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16608-1-PBS in a different storage buffer formulation.

APOF Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 16608-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Apo F, Apo-F, apolipoprotein F, LTIP

---

Product Information

16608-1-PBS targets APOF in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9824

  Product name: Recombinant human APOF protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 37-326 aa of BC026257

  Sequence: TSYGKQTNVLMHFPLSLESQTPSSDPLSCQFLHPKSLPGFSHMAPLPKFLVSLALRNALEEAGCQADVWALQLQLYRQGGVNATQVLIQHLRGLQKGRSTERNVSVEALASALQLLAREQQSTGRVGRSLPTEDCENEKEQAVHNVVQLLPGVGTFYNLGTALYYATQNCLGKARERGRDGAIDLGYDLLMTMAGMSGGPMGLAISAALKPALRSGVQQLIQYYQDQKDANISQPETTKEGLRAISDVSDLEETTTLASFISEVVSSAPYWGWAIIKSYDLDPGAGSLEI

• Full Name: apolipoprotein F
• Calculated Molecular Weight: 326 aa, 35 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC026257
• Gene Symbol: APOF
• Gene ID (NCBI): 319
• RRID: AB_2058253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13790
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

APOF protein, full name Apolipoprotein F, also known as Lipid transfer inhibitor protein (LTIP), belongs to the apolipoprotein F family. This protein is primarily expressed by the liver and secreted into the plasma. Its core function is to act as a cholesterol transport regulator, modulating the transport of cholesterol among plasma lipoproteins by inhibiting the activity of cholesteryl ester transfer protein (CETP). APOF is initially synthesized as a precursor protein with a theoretical molecular weight of approximately 35.4 kDa. After processing, the mature peptide has a molecular weight of about 17.4 kDa. However, in plasma, due to modifications such as glycosylation, the apparent molecular weight detected experimentally is typically around 33-35 kDa.