APOL2 Polyclonal antibody

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  WB analysis of A549 using 25925-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25925-1-AP (APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25925-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25925-1-AP (APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25925-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25925-1-AP (APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 25925-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25925-1-AP (APOL2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 25925-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25925-1-AP (APOL2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 25925-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APOL2 antibody (25925-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

APOL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25925-1-AP

Publications(4)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Apolipoprotein L-II, ApoL-II, APOL3, APOL II

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells, HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

25925-1-AP targets APOL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7622

  Product name: Recombinant human APOL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-337 aa of BC004395

  Sequence: MNPESSIFIEDYLKYFQDQVSRENLLQLLTDDEAWNGFVAAAELPRDEADELRKALNKLASHMVMKDKNRHDKDQQHRQWFLKEFPRLKRELEDHIRKLRALAEEVEQVHRGTTIANVVSNSVGTTSGILTLLGLGLAPFTEGISFVLLDTGMGLGAAAAVAGITCSVVELVNKLRARAQARNLDQSGTNVAKVMKEFVGGNTPNVLTLVDNWYQVTQGIGRNIRAIRRARANPQLGAYAPPPHVIGRISAEGGEQVERVVEGPAQAMSRGTMIVGAATGGILLLLDVVSLAYESKHLLEGAKSESAEELKKRAQELEGKLNFLTKIHEMLQPGQDQ

• Full Name: apolipoprotein L, 2
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC004395
• Gene Symbol: APOL2
• Gene ID (NCBI): 23780
• RRID: AB_2880297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BQE5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Chem Commun (Camb); Competitive affinity-based proteome profiling and imaging to reveal potential cellular targets of betulinic acid.; Authors - Haijun Guo ; KD Validated
human	WB	Aging (Albany NY); TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs); Authors - Jianyun Liu
human,mouse	WB	Nat Chem Biol; A natural small molecule alleviates liver fibrosis by targeting apolipoprotein L2; Authors - Lu Gan
human	WB	Adv Sci (Weinh); APOL2 Stabilizes Ku80 to Confer NHEJ-Mediated Radioresistance in Gastric Cancer; Authors - Dan Zu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Melissa (Verified Customer) (07-28-2022)

This antibody worked well in Huh7 cells. The antibody was used to blot for APOL2 after co-IP for another protein of interest.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Huh7 cells

APOL2 Antibody Western Blot validation (1:1000 dilution) in Huh7 cells (Cat no:25925-1-AP)