• Featured Product
  • KD/KO Validated

APOL2 Polyclonal antibody

APOL2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25925-1-AP

Synonyms

APOL II, APOL2, APOL3, Apolipoprotein L II, apolipoprotein L, 2, Apolipoprotein L2



Tested Applications

Positive WB detected inA549 cells, A431 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25925-1-AP is APOL2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOL2 fusion protein Ag7622
Full Name apolipoprotein L, 2
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC004395
Gene Symbol APOL2
Gene ID (NCBI) 23780
RRIDAB_2880297
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for APOL2 antibody 25925-1-APDownload protocol
IHC protocol for APOL2 antibody 25925-1-APDownload protocol
IF protocol for APOL2 antibody 25925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Chem Commun (Camb)

Competitive affinity-based proteome profiling and imaging to reveal potential cellular targets of betulinic acid.

Authors - Haijun Guo
  • KD Validated
humanWB

Aging (Albany NY)

TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs)

Authors - Jianyun Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Melissa (Verified Customer) (07-28-2022)

This antibody worked well in Huh7 cells. The antibody was used to blot for APOL2 after co-IP for another protein of interest.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Huh7 cells
APOL2 Antibody Western Blot validation (1:1000 dilution) in Huh7 cells (Cat no:25925-1-AP)