Tested Applications
| Positive WB detected in | A431 cells, A549 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human lung cancer tissue, human stomach cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25925-1-AP targets APOL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7622 Product name: Recombinant human APOL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-337 aa of BC004395 Sequence: MNPESSIFIEDYLKYFQDQVSRENLLQLLTDDEAWNGFVAAAELPRDEADELRKALNKLASHMVMKDKNRHDKDQQHRQWFLKEFPRLKRELEDHIRKLRALAEEVEQVHRGTTIANVVSNSVGTTSGILTLLGLGLAPFTEGISFVLLDTGMGLGAAAAVAGITCSVVELVNKLRARAQARNLDQSGTNVAKVMKEFVGGNTPNVLTLVDNWYQVTQGIGRNIRAIRRARANPQLGAYAPPPHVIGRISAEGGEQVERVVEGPAQAMSRGTMIVGAATGGILLLLDVVSLAYESKHLLEGAKSESAEELKKRAQELEGKLNFLTKIHEMLQPGQDQ Predict reactive species |
| Full Name | apolipoprotein L, 2 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC004395 |
| Gene Symbol | APOL2 |
| Gene ID (NCBI) | 23780 |
| RRID | AB_2880297 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BQE5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Apolipoprotein L2 (APOL2) is a member of the apolipoprotein L gene family. Apolipoprotein L (APOL) proteins belong to the high-density lipoprotein family, which plays a central role in cholesterol transport. APOL2, mainly localized at the endoplasmic reticulum, is implicated in cholesterol biosynthesis and trafficking and mediates cell death induced by interferon-gamma or viral infection.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for APOL2 antibody 25925-1-AP | Download protocol |
| IHC protocol for APOL2 antibody 25925-1-AP | Download protocol |
| WB protocol for APOL2 antibody 25925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The APOL2 antibody (Cat# 25925-1-AP) has 4 citations published in Nature Chemical Biology, Advanced Science, Chemical Communications, and Aging. The associated research fields span liver fibrosis, gastric cancer radioresistance, proteome profiling of betulinic acid targets, and osteogenic differentiation of mesenchymal stem cells. All citations utilized the antibody for Western blot applications, with species tested including human and mouse.
| Species | Application | Title |
|---|---|---|
Nat Chem Biol A natural small molecule alleviates liver fibrosis by targeting apolipoprotein L2 | ||
Adv Sci (Weinh) APOL2 Stabilizes Ku80 to Confer NHEJ-Mediated Radioresistance in Gastric Cancer | ||
Chem Commun (Camb) Competitive affinity-based proteome profiling and imaging to reveal potential cellular targets of betulinic acid.
| ||
Aging (Albany NY) TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs) |
Reviews
What customers say
Both reviewers used this antibody for Western Blot at a 1:1000 dilution. One user reported fairly high background but noted a distinct double band at the correct molecular weight in HepG2 cells. The other user found it performed well in Huh7 cells, specifically for blotting APOL2 after a co-immunoprecipitation experiment. Overall, the antibody is considered functional for WB with acceptable specificity, though some background may be observed.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anna (Verified Customer) (02-05-2026) | Fairly high background, but has distinct double band at the correct MW.
|
Melissa (Verified Customer) (07-28-2022) | This antibody worked well in Huh7 cells. The antibody was used to blot for APOL2 after co-IP for another protein of interest.
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