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APOL2 Polyclonal antibody, PBS Only (25925-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of A549 using 25925-1-AP (same clone as 25925-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 25925-1-AP (APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25925-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 25925-1-AP (same clone as 25925-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 25925-1-AP (APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25925-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 25925-1-AP (same clone as 25925-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 25925-1-AP (APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25925-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 25925-1-AP (same clone as 25925-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25925-1-AP (APOL2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25925-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 25925-1-AP (same clone as 25925-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25925-1-AP (APOL2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25925-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 25925-1-AP (same clone as 25925-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APOL2 antibody (25925-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 25925-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

APOL2 Polyclonal antibody, PBS Only

Name: APOL2 Polyclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 25925-1-PBS-100UG

APOL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 25925-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

APOL II, APOL3, ApoL-II, Apolipoprotein L-II

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

25925-1-PBS targets APOL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7622 Product name: Recombinant human APOL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-337 aa of BC004395 Sequence: MNPESSIFIEDYLKYFQDQVSRENLLQLLTDDEAWNGFVAAAELPRDEADELRKALNKLASHMVMKDKNRHDKDQQHRQWFLKEFPRLKRELEDHIRKLRALAEEVEQVHRGTTIANVVSNSVGTTSGILTLLGLGLAPFTEGISFVLLDTGMGLGAAAAVAGITCSVVELVNKLRARAQARNLDQSGTNVAKVMKEFVGGNTPNVLTLVDNWYQVTQGIGRNIRAIRRARANPQLGAYAPPPHVIGRISAEGGEQVERVVEGPAQAMSRGTMIVGAATGGILLLLDVVSLAYESKHLLEGAKSESAEELKKRAQELEGKLNFLTKIHEMLQPGQDQ Predict reactive species
Full Name	apolipoprotein L, 2
Calculated Molecular Weight	37 kDa
Observed Molecular Weight	37 kDa
GenBank Accession Number	BC004395
Gene Symbol	APOL2
Gene ID (NCBI)	23780
RRID	AB_2880297
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9BQE5
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Apolipoprotein L2 (APOL2) is a member of the apolipoprotein L gene family. Apolipoprotein L (APOL) proteins belong to the high-density lipoprotein family, which plays a central role in cholesterol transport. APOL2, mainly localized at the endoplasmic reticulum, is implicated in cholesterol biosynthesis and trafficking and mediates cell death induced by interferon-gamma or viral infection.