APOL4 Polyclonal antibody

APOL4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10564-1-AP

Synonyms

APOL IV, APOL4, Apolipoprotein L IV, apolipoprotein L, 4, Apolipoprotein L4, APOLIV

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Validation Data Gallery

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  WB analysis using 10564-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10564-1-AP (APOL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10564-1-AP

  IP result of anti-APOL4 (IP:10564-1-AP, 4ug; Detection:10564-1-AP 1:2000) with HepG2 cells lysate 4000ug.

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  IHC staining of human liver cancer using 10564-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 10564-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 10564-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 10564-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 10564-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10564-1-AP (APOL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, U-87 MG cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10564-1-AP targets APOL4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APOL4 fusion protein Ag0849
• Full Name: apolipoprotein L, 4
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC006276
• Gene Symbol: APOL4
• Gene ID (NCBI): 80832
• RRID: AB_2242901
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.