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APOM Polyclonal antibody, PBS Only (12817-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 12817-1-AP (same clone as 12817-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12817-1-AP (APOM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.
WB analysis of human plasma using 12817-1-AP (same clone as 12817-1-PBS)
human plasma were subjected to SDS PAGE followed by western blot with 12817-1-AP (APOM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 12817-1-AP (same clone as 12817-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12817-1-AP (APOM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 12817-1-AP (same clone as 12817-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12817-1-AP (APOM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 12817-1-AP (same clone as 12817-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12817-1-AP (APOM Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 12817-1-AP (same clone as 12817-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12817-1-AP (APOM Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 12817-1-AP (same clone as 12817-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOM antibody (12817-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

APOM Polyclonal antibody, PBS Only

Name: APOM Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12817-1-PBS

APOM Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12817-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12817-1-PBS targets APOM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3137 Product name: Recombinant human APOM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC020683 Sequence: MFHQIWAALLYFYGIILNSIYQCPEHSQLTTLGVDGKEFPEVHLGQWYFIAGAAPTKEELATFDPVDNIVFNMAAGSAPMQLHLRATIRMKDGLCVPRKWIYHLTEGSTDLRTEGRPDMKTELFSSSCPGGIMLNETGQGYQRFLLYNRSPHPPEKCVEEFKSLTSCLDSKAFLLTPRNQEACELSNN Predict reactive species
Full Name	apolipoprotein M
Calculated Molecular Weight	21 kDa
Observed Molecular Weight	21-25 kDa
GenBank Accession Number	BC020683
Gene Symbol	APOM
Gene ID (NCBI)	55937
RRID	AB_2877884
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95445
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

APOM (Apolipoprotein M) is a member of the lipocalin superfamily and is predominantly associated with high-density lipoprotein (HDL). APOM is synthesized in the kidneys, liver, and to a minor extent also by adipocytes. In the circulation, APOM binds primarily to high-density lipoproteins (HDL) but can also be associated with other lipoproteins, such as low-density lipoproteins (LDL), very-low-density lipoproteins (VLDL), and chylomicrons (PMID: 34482091).