APPL2 Polyclonal antibody

APPL2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14294-1-AP

Synonyms

Dip13-beta, DIP13B, Dip13 beta, DCC-interacting protein 13-beta, Adapter protein containing PH domain, PTB domain and leucine zipper motif 2

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Validation Data Gallery

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  WB analysis of BxPC-3 using 14294-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14294-1-AP (APPL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 14294-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14294-1-AP (APPL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of BxPC-3 using 14294-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed BxPC-3 cells using 14294-1-AP (APPL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: BxPC-3 cells
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

14294-1-AP targets APPL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APPL2 fusion protein Ag5626
• Full Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC033731
• Gene Symbol: APPL2
• Gene ID (NCBI): 55198
• RRID: AB_2878041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Cachexia Sarcopenia Muscle; Physical exercise attenuates age-related muscle atrophy and exhibits anti-ageing effects via the adiponectin receptor 1 signalling; Authors - Yuan-Li Chen
human	WB	J Immunol; Long Noncoding RNA HAFML Promotes Migration and Invasion of Rheumatoid Fibroblast-like Synoviocytes; Authors - Siqi Xu
mouse	IF	J Cell Sci; GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region; Authors - Paroma Chatterjee
mouse	IF	bioRxiv; GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region; Authors - Paroma Chatterjee