APPL2 Polyclonal antibody, PBS Only

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• APPL2 Polyclonal antibody, PBS Only (14294-1-PBS)

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  WB analysis of BxPC-3 using 14294-1-AP (same clone as 14294-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14294-1-AP (APPL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 14294-1-AP (same clone as 14294-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14294-1-AP (APPL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14294-1-AP (same clone as 14294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14294-1-AP (same clone as 14294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 14294-1-AP (same clone as 14294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14294-1-AP (same clone as 14294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 14294-1-AP (same clone as 14294-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using APPL2 antibody (14294-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

APPL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14294-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Dip13-beta, DIP13B, Dip13 beta, DCC-interacting protein 13-beta, Adapter protein containing PH domain, PTB domain and leucine zipper motif 2

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Product Information

14294-1-PBS targets APPL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5626

  Product name: Recombinant human APPL2 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 314-664 aa of BC033731

  Sequence: RGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKVTASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

• Full Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC033731
• Gene Symbol: APPL2
• Gene ID (NCBI): 55198
• RRID: AB_2878041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NEU8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.