ARD1A Polyclonal antibody

ARD1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 14803-1-AP

Synonyms

N-alpha-acetyltransferase 10, Naa10p, NAA10, hARD1, EC:2.3.1.255

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Validation Data Gallery

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  WB analysis using 14803-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14803-1-AP (ARD1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14803-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14803-1-AP (ARD1A Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14803-1-AP

  IP result of anti-ARD1A (IP:14803-1-AP, 4ug; Detection:14803-1-AP 1:500) with MCF-7 cells lysate 3200ug.

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  IHC staining of human breast cancer using 14803-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14803-1-AP (ARD1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14803-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14803-1-AP (ARD1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14803-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARD1A antibody (14803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HL-60 cells, mouse spleen tissue, HeLa cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14803-1-AP targets ARD1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARD1A fusion protein Ag6519
• Full Name: ARD1 homolog A, N-acetyltransferase (S. cerevisiae)
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC000308
• Gene Symbol: ARD1A
• Gene ID (NCBI): 8260
• RRID: AB_1557262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Elife; Naa12 compensates for Naa10 in mice in the amino-terminal acetylation pathway.; Authors - Hyae Yon Kweon
Yeast	WB	Yeast; Proteomic and genomic characterization of a yeast model for Ogden syndrome.; Authors - Max J Dörfel
		Methods Enzymol; Peptide CoA conjugates for in situ proteomics profiling of acetyltransferase activities; Authors - Jürgen Eirich

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zehua (Verified Customer) (07-09-2018)

Samples from Proteintech. Works very well for WB.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF-7