Arfaptin-1 Polyclonal antibody

Arfaptin-1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17726-1-AP

Synonyms

Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521



Tested Applications

Positive WB detected inBxPC-3 cells, HCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, Raw 264.7 cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

17726-1-AP targets Arfaptin-1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Arfaptin-1 fusion protein Ag12122
Full Name ADP-ribosylation factor interacting protein 1
Calculated molecular weight 373 aa, 42 kDa
Observed molecular weight 42 kDa, 38 kDa, 22 kDa
GenBank accession numberBC103759
Gene symbol ARFIP1
Gene ID (NCBI) 27236
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
IHC protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
IF protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Authors - Liyuan Zhang