ARH3 Polyclonal antibody, PBS Only

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• ARH3 Polyclonal antibody, PBS Only (16504-1-PBS)

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  WB analysis using 16504-1-AP (same clone as 16504-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 16504-1-AP (same clone as 16504-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 16504-1-AP (same clone as 16504-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 16504-1-AP (same clone as 16504-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 16504-1-AP (same clone as 16504-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 16504-1-AP (same clone as 16504-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16504-1-AP (same clone as 16504-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 16504-1-AP (same clone as 16504-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 16504-1-AP (same clone as 16504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 16504-1-AP (same clone as 16504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16504-1-AP (same clone as 16504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16504-1-AP (same clone as 16504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16504-1-AP (same clone as 16504-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 16504-1-AP (ARH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

ARH3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 16504-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

16504-1-PBS targets ARH3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9683

  Product name: Recombinant human ADPRHL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-363 aa of BC014169

  Sequence: MAAAAMAAAAGGGAGAARSLSRFRGCLAGALLGDCVGSFYEAHDTVDLTSVLRHVQSLEPDPGTPGSERTEALYYTDDTAMARALVQSLLAKEAFDEVDMAHRFAQEYKKDPDRGYGAGVVTVFKKLLNPKCRDVFEPARAQFNGKGSYGNGGAMRVAGISLAYSSVQDVQKFARLSAQLTHASSLGYNGAILQALAVHLALQGESSSEHFLKQLLGHMEDLEGDAQSVLDARELGMEERPYSSRLKKIGELLDQASVTREEVVSELGNGIAAFESVPTAIYCFLRCMEPDPEIPSAFNSLQRTLIYSISLGGDTDTIATMAGAIAGAYYGMDQVPESWQQSCEGYEETDILAQSLHRVFQKS

• Full Name: ADP-ribosylhydrolase like 2
• Calculated Molecular Weight: 363 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC014169
• Gene Symbol: ARH3
• Gene ID (NCBI): 54936
• RRID: AB_2224919
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NX46
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ARH3 can be detected as two bands between 39 and 40 kDa.(PMID: 16278211/32636369)