ARHGAP12 Polyclonal antibody, PBS Only

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• ARHGAP12 Polyclonal antibody, PBS Only (17855-1-PBS)

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  IHC staining of mouse cerebellum using 17855-1-AP (same clone as 17855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17855-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 17855-1-AP (same clone as 17855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17855-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 17855-1-AP (same clone as 17855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17855-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17855-1-AP (same clone as 17855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17855-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 17855-1-AP (same clone as 17855-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using ARHGAP12 antibody (17855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17855-1-PBS in a different storage buffer formulation.

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  IF Staining of rat kidney using 17855-1-AP (same clone as 17855-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat kidney tissue using ARHGAP12 antibody (17855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17855-1-PBS in a different storage buffer formulation.

ARHGAP12 Polyclonal Antibody for IHC, IF-P, Indirect ELISA

Cat No. 17855-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, Indirect ELISA

Synonyms

Rho GTPase-activating protein 12, Rho-type GTPase-activating protein 12

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Product Information

17855-1-PBS targets ARHGAP12 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12309

  Product name: Recombinant human ARHGAP12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 486-841 aa of BC094719

  Sequence: AVLQGSSLLFTKTQGSSTSWFGSNQSKPEFTVDLKGATIEMASKDKSSKKNVFELKTRQGTELLIQSDNDTVINDWFKVLSSTINNQAVETDEGIEEEIPDSPGIEKHDKEKEQKDPKKLRSFKVSSIDSSEQKKTKKNLKKFLTRRPTLQAVREKGYIKDQVFGSNLANLCQRENGTVPKFVKLCIEHVEEHGLDIDGIYRVSGNLAVIQKLRFAVNHDEKLDLNDSKWEDIHVITGALKMFFRELPEPLFTFNHFNDFVNAIKQEPRQRVAAVKDLIRQLPKPNQDTMQILFRHLRRVIENGEKNRMTYQSIAIVFGPTLLKPEKETGNIAVHTVYQNQIVELILLELSSIFGR

• Full Name: Rho GTPase activating protein 12
• Calculated Molecular Weight: 841 aa, 96 kDa
• GenBank Accession Number: BC094719
• Gene Symbol: ARHGAP12
• Gene ID (NCBI): 94134
• RRID: AB_3741937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IWW6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ARHGAP12 belongs to the Rho GTPase-activating protein (RhoGAP) family. RhoGAP is a key regulatory factor of the small GTPase Rho family and serves as a molecular switch that regulates various cellular processes. ARHGAP12 is involved in suppressing tumor formation by regulating cell invasion and adhesion.