ARHGAP21 Polyclonal antibody

ARHGAP21 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22183-1-AP

Synonyms

ARHGAP21, KIAA1424



Tested Applications

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inhuman ovary tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22183-1-AP is ARHGAP21 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP21 fusion protein Ag16515
Full Name Rho GTPase activating protein 21
Calculated Molecular Weight 1957 aa, 217 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberBC118915
Gene Symbol ARHGAP21
Gene ID (NCBI) 57584
RRIDAB_2879018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARHGAP21 antibody 22183-1-APDownload protocol
IHC protocol for ARHGAP21 antibody 22183-1-APDownload protocol
IF protocol for ARHGAP21 antibody 22183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Phosphorylation-dependent recognition of diverse protein targets by the cryptic GK domain of MAGI MAGUKs

Authors - Meng Zhang
  • KD Validated
humanWB,IF

Elife

PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex.

Authors - Arman Javadi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-03-2019)

first 2 lanes are whole cell lysate. ARHGAP21doesn't show up prominently but does show up in last 2 lanes which are purified biotinylated proteins surrounding out protein of interest. This seems to indicate that the antibody works but just that the cells we use express levels that are below the level of detection unless it is enriched (such as with our purification protocol)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
ARHGAP21 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:22183-1-AP)