CoraLite® Plus 488-conjugated ARHGAP21 Polyclonal antibody

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  FC experiment of HepG2 using CL488-22183

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Arhgap21 Polyclonal Antibody (CL488-22183)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

ARHGAP21 Polyclonal Antibody for FC (Intra)

Cat No. CL488-22183

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

FC (Intra)

Synonyms

ARHGAP10, KIAA1424, Rho GTPase-activating protein 10, Rho-type GTPase-activating protein 21

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Tested Applications

• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-22183 targets ARHGAP21 in FC (Intra) applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16515

  Product name: Recombinant human ARHGAP21 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1598-1957 aa of BC118915

  Sequence: LDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGTKAEFHPCL

• Full Name: Rho GTPase activating protein 21
• Calculated Molecular Weight: 1957 aa, 217 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC118915
• Gene Symbol: ARHGAP21
• Gene ID (NCBI): 57584
• RRID: AB_3672740
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T5U3
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.