ARHGAP21 Polyclonal antibody, PBS Only

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• ARHGAP21 Polyclonal antibody, PBS Only (22183-1-PBS)

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  WB analysis using 22183-1-AP (same clone as 22183-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22183-1-AP (ARHGAP21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22183-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 22183-1-AP (same clone as 22183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 22183-1-AP (ARHGAP21 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22183-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 22183-1-AP (same clone as 22183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22183-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 22183-1-AP (same clone as 22183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22183-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22183-1-AP (same clone as 22183-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARHGAP21 antibody (22183-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22183-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ARHGAP21 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 22183-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Rho-type GTPase-activating protein 21, Rho GTPase-activating protein 10, KIAA1424, ARHGAP10

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Product Information

22183-1-PBS targets ARHGAP21 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16515

  Product name: Recombinant human ARHGAP21 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1598-1957 aa of BC118915

  Sequence: LDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGTKAEFHPCL

• Full Name: Rho GTPase activating protein 21
• Calculated Molecular Weight: 1957 aa, 217 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC118915
• Gene Symbol: ARHGAP21
• Gene ID (NCBI): 57584
• RRID: AB_2879018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T5U3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.