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ARHGDIB Polyclonal antibody

ARHGDIB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16122-1-AP

Synonyms

ARHGDIB, D4, GDIA2, GDID4, Ly GDI, LYGDI, RAP1GN1, Rho GDI 2, Rho GDI beta, RhoGDI2



Tested Applications

Positive WB detected inHL-60 cells, K-562 cells, rat spleen tissue, Jurkat cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16122-1-AP targets ARHGDIB in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGDIB fusion protein Ag9078
Full Name Rho GDP dissociation inhibitor (GDI) beta
Calculated Molecular Weight 201 aa, 23 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC009200
Gene Symbol ARHGDIB
Gene ID (NCBI) 397
RRIDAB_2059596
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.

Protocols

Product Specific Protocols
WB protocol for ARHGDIB antibody 16122-1-APDownload protocol
IHC protocol for ARHGDIB antibody 16122-1-APDownload protocol
IP protocol for ARHGDIB antibody 16122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Metab

Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression

Authors - Lin Qian
humanWB

Nat Immunol

Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.

Authors - Jing Geng
humanWB

Mol Cancer

KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1.

Authors - Lei Liu
humanWB

Proteomics

Changes in the proteomic profile during the differential polarization status of the human monocyte-derived macrophage THP-1 cell line.

Authors - Fan Zhang
humanWB,IHC

Cancer Biomark

Systematic investigation of biomarker-like role of ARHGDIB in breast cancer.

Authors - Xiaonan Wang
  • KD Validated
humanWB

DNA Cell Biol

Rho GDP Dissociation Inhibitor (GDI) Beta Promotes Cell Proliferation and Invasion by Modulating the AKT Pathway in Hepatocellular Carcinoma.

Authors - Yang Fang