ARHGEF1 Polyclonal antibody

ARHGEF1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11363-1-AP

Synonyms

ARHGEF1, GEF1, LBCL2, LSC, P115 RHOGEF, p115RhoGEF, SUB1.5



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, human spleen tissue, Jurkat cells, K-562 cells, mouse spleen tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11363-1-AP targets ARHGEF1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGEF1 fusion protein Ag1905
Full Name Rho guanine nucleotide exchange factor (GEF) 1
Calculated Molecular Weight 912 aa, 102 kDa
Observed Molecular Weight 102-110 kDa
GenBank Accession NumberBC011726
Gene Symbol ARHGEF1
Gene ID (NCBI) 9138
RRIDAB_2059741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARHGEF1 antibody 11363-1-APDownload protocol
IHC protocol for ARHGEF1 antibody 11363-1-APDownload protocol
IF protocol for ARHGEF1 antibody 11363-1-APDownload protocol
IP protocol for ARHGEF1 antibody 11363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.

Authors - Silvana Konermann
humanWB

Nat Metab

Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression

Authors - Lin Qian
humanWB

Mol Biol Cell

The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα.

Authors - P V Ryder
mouseCoIP,WB

Nat Commun

Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis

Authors - Yaping Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Udesh (Verified Customer) (12-29-2023)

Worked well for IP using 6ug Ab and WB 1:1500

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Glioma
FH

can (Verified Customer) (12-17-2019)

Very good antibody!

  • Applications: Immunoprecipitation,
  • Cell Tissue Type: blood vascular cell
FH

Ni (Verified Customer) (09-26-2019)

Very successful in immunoprecipitating p115RhoGEF from transfected cells.

  • Applications: Immunoprecipitation,
  • Primary Antibody Dilution: 2 ug /reaction