ARHGEF11 Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP01063-2

  Sandwich ELISA standard curve of MP01063-2, Human ARHGEF11 Recombinant Matched Antibody Pair - PBS only. 84149-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33104. 84149-1-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

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  Sample test of MP01063-2

  The mean ARHGEF11 concentration was determined to be 4.0 ng/mL in HEK-293T cell extract based on a 3.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP01063-2

  Cytometric bead array standard curve of MP01063-2, ARHGEF11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84149-3-PBS. Detection antibody: 84149-1-PBS. Standard: Ag33104. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84149-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 84149-3-PBS against Human ARHGEF11 was performed. The affinity constant is below 1 pM.

ARHGEF11 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84149-3-PBS

Clone No.241427D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

PDZ RHOGEF, KIAA0380, GTRAP48, 241427D6

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Product Information

84149-3-PBS targets ARHGEF11 as part of a matched antibody pair:

MP01063-2: 84149-3-PBS capture and 84149-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag33104

  Product name: Recombinant human ARHGEF11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1223-1522 aa of

  Sequence: ETQAAQEPEDDLTPTPSVISVTSHPWDPGSPGQAPPGGEGDNTQLAGLEGERPEQEDMGLCSLEHLPPRTRNSGIWESPELDRNLAEDASSTEAAGGYKVVRKAEVAGSKVVPALPESGQSEPGPPEVEGGTKATGNCFYVSMPSGPPDSSTDHSEAPMSPPQPDSLPAGQTEPQPQLQGGNDDPRRPSRSPPSLALRDVGMIFHTIEQLTLKLNRLKDMELAHRELLKSLGGESSGGTTPVGSFHTEAARWTDGSLSPPAKEPLASDSRNSHELGPCPEDGSDAPLEDSTADAAASPGP

• Full Name: Rho guanine nucleotide exchange factor (GEF) 11
• Calculated Molecular Weight: 168 kDa
• Gene Symbol: ARHGEF11
• Gene ID (NCBI): 9826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15085
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.