ARHGEF11 Recombinant monoclonal antibody

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  WB analysis using 84149-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84149-4-RR (ARHGEF11 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84149-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84149-4-RR against Human ARHGEF11 were performed. The affinity constant is 0.742 nM.

ARHGEF11 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84149-4-RR

Clone No.241427G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PDZ RHOGEF, KIAA0380, GTRAP48, 241427G2

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Tested Applications

• Positive WB detected in: HEK-293T cells, HuH-7 cells, COLO 205 cells, MDA-MB-453 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84149-4-RR targets ARHGEF11 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag33104

  Product name: Recombinant human ARHGEF11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1223-1522 aa of

  Sequence: ETQAAQEPEDDLTPTPSVISVTSHPWDPGSPGQAPPGGEGDNTQLAGLEGERPEQEDMGLCSLEHLPPRTRNSGIWESPELDRNLAEDASSTEAAGGYKVVRKAEVAGSKVVPALPESGQSEPGPPEVEGGTKATGNCFYVSMPSGPPDSSTDHSEAPMSPPQPDSLPAGQTEPQPQLQGGNDDPRRPSRSPPSLALRDVGMIFHTIEQLTLKLNRLKDMELAHRELLKSLGGESSGGTTPVGSFHTEAARWTDGSLSPPAKEPLASDSRNSHELGPCPEDGSDAPLEDSTADAAASPGP

• Full Name: Rho guanine nucleotide exchange factor (GEF) 11
• Calculated Molecular Weight: 168 kDa
• Observed Molecular Weight: 220 kDa
• Gene Symbol: ARHGEF11
• Gene ID (NCBI): 9826
• RRID: AB_3671714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15085
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGEF11 (Rho guanine nucleotide exchange factor 11) is a specific guanine nucleotide exchange factor (GEF) for RhoA, which is a critical regulator of actin cytoskeleton dynamics and organization of dendritic spines and inhibitor of spine maintenance (PMID: 28036092). ARHGEF11 has been proven to promote tumor metastasis in glioblastoma and ovarian carcinoma (PMID: 33122451).