ARHGEF11 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84149-4-RR (same clone as 84149-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84149-4-RR (ARHGEF11 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84149-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01063-3

  Cytometric bead array standard curve of MP01063-3, ARHGEF11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84149-4-PBS. Detection antibody: 84149-2-PBS. Standard: Ag33104. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84149-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 84149-4-PBS against Human ARHGEF11 was performed. The affinity constant is 0.707 nM.

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  Affinity and Kinetic Characterization of 84149-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84149-4-RR against Human ARHGEF11 were performed. The affinity constant is 0.742 nM.

ARHGEF11 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 84149-4-PBS

Clone No.241427G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

PDZ RHOGEF, KIAA0380, GTRAP48, 241427G2

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Product Information

84149-4-PBS targets ARHGEF11 as part of a matched antibody pair:

MP01063-3: 84149-4-PBS capture and 84149-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ARHGEF11 fusion protein Ag33104
• Full Name: Rho guanine nucleotide exchange factor (GEF) 11
• Calculated Molecular Weight: 168 kDa
• Observed Molecular Weight: 220 kDa
• Gene Symbol: ARHGEF11
• Gene ID (NCBI): 9826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15085
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ARHGEF11 (Rho guanine nucleotide exchange factor 11) is a specific guanine nucleotide exchange factor (GEF) for RhoA, which is a critical regulator of actin cytoskeleton dynamics and organization of dendritic spines and inhibitor of spine maintenance (PMID: 28036092). ARHGEF11 has been proven to promote tumor metastasis in glioblastoma and ovarian carcinoma (PMID: 33122451).