ARHGEF28 Polyclonal antibody, PBS Only

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• ARHGEF28 Polyclonal antibody, PBS Only (17144-1-PBS)

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  WB analysis of HeLa using 17144-1-AP (same clone as 17144-1-PBS)

  WB result of ARHGEF28 antibody (17144-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARHGEF28 transfected HeLa cells. This data was developed using the same antibody clone with 17144-1-PBS in a different storage buffer formulation.

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  WB analysis using 17144-1-AP (same clone as 17144-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17144-1-AP (RGNEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17144-1-PBS in a different storage buffer formulation.

ARHGEF28 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 17144-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

RGNEF, ARG28, p190 RhoGEF, p190RhoGEF, p190-RhoGEF

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Product Information

17144-1-PBS targets ARHGEF28 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10915

  Product name: Recombinant human RGNEF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC012946

  Sequence: MIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSL

• Full Name: Rho-guanine nucleotide exchange factor
• Calculated Molecular Weight: 192 kDa
• Observed Molecular Weight: 192 kDa
• GenBank Accession Number: BC012946
• Gene Symbol: RGNEF
• Gene ID (NCBI): 64283
• RRID: AB_3669262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N1W1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ARHGEF28, also known as RGNEF or p190RhoGEF, is a 190 kDa RNA binding protein (RBP) that contains a Dbl/PH domain capable of RhoA activation. ARHGEF28 interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates.