ARHGEF5 Polyclonal antibody

ARHGEF5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11379-1-AP

Synonyms

ARHGEF5, GEF5, Oncogene TIM, P60, p60 TIM, TIM, TIM1

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 11379-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11379-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11379-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11379-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11379-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 11379-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11379-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11379-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 11379-1-AP

  IP result of anti-ARHGEF5 (IP:11379-1-AP, 4ug; Detection:11379-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human lung cancer using 11379-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11379-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11379-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11379-1-AP (ARHGEF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: MCF7 cells, HEK-293 cells, HeLa cells, human kidney tissue, human liver tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11379-1-AP targets ARHGEF5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARHGEF5 fusion protein Ag1892
• Full Name: Rho guanine nucleotide exchange factor (GEF) 5
• Calculated Molecular Weight: 177 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC010046
• Gene Symbol: ARHGEF5
• Gene ID (NCBI): 7984
• RRID: AB_2060050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB, IF	J Biol Chem; Odontogenic ameloblast-associated protein (ODAM) Mediates Junctional Epithelium Attachment to Tooth via Integrin-ODAM-Rho guanine nucleotide exchange factor 5 (ARHGEF5)-Ras homolog gene family member A (RhoA) Signaling.; Authors - Hye-Kyung Lee
human	IHC	Oncol Rep; Co-expression of Rho guanine nucleotide exchange factor 5 and Src associates with poor prognosis of patients with resected non-small cell lung cancer.; Authors - He Ping P
human	WB	Cell Biochem Funct; ODAM inhibits RhoA-dependent invasion in breast cancer.; Authors - Hye-Kyung Lee