ARID1B Recombinant antibody, PBS Only

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  IF Staining of HepG2 using 82979-1-RR (same clone as 82979-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARID1B antibody (82979-1-RR, Clone: 230222G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82979-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82979-1-RR (same clone as 82979-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARID1B antibody (82979-1-RR, Clone: 230222G4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82979-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 82979-1-RR (same clone as 82979-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-HUMAN ARID1B (82979-1-RR, Clone:230222G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP)(blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82979-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82979-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82979-1-RR against Human ARID1B were performed. The affinity constant is 0.606 nM.

ARID1B Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 82979-1-PBS

Clone No.230222G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

hOsa2, ELD/OSA1, DAN15, BRIGHT, BAF250b

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Product Information

82979-1-PBS targets ARID1B in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ARID1B fusion protein Ag30643
• Full Name: AT rich interactive domain 1B (SWI1-like)
• Calculated Molecular Weight: 236 kDa
• GenBank Accession Number: NM_017519
• Gene Symbol: ARID1B
• Gene ID (NCBI): 57492
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NFD5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.