CoraLite® Plus 488-conjugated ARID4A Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated ARID4A Recombinant monoclonal antibody (CL488-83489)

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  FC experiment of U2OS using CL488-83489

  1x10^6 U2OS cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated ARID4A Recombinant monoclonal antibody (CL488-83489, Clone:240436A4)(red), or 0.8 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

ARID4A Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL488-83489

Clone No.240436A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

240436A4, ARID 4A, ARID domain-containing protein 4A, AT-rich interactive domain-containing protein 4A, RBBP 1

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Tested Applications

• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.8 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83489 targets ARID4A in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag33114

  Product name: Recombinant human ARID4A protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 900-1105 aa of NM_002892.3

  Sequence: FEQHFERENEGMPSLIAESNQCIQQLTSERFDSPAEETVNIPLKEDEDAMPLIGPETLVCHEVDLDDLDEKDKTSIEDVAVESSESNSLVSIPPALPPVVQHNFSVASPLTLSQDESRSVKSESDITIEVDSIAEESQEGLCERESANGFETNVASGTCSIIVQERESREKGQKRPSDGNSGLMAKKQKRTPKRTSAAAKNEKNGT

• Full Name: AT rich interactive domain 4A (RBP1-like)
• Calculated Molecular Weight: 143 kDa
• Observed Molecular Weight: 205 kDa
• GenBank Accession Number: NM_002892.3
• Gene Symbol: ARID4A
• Gene ID (NCBI): 5926
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29374
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARID4A (AT-rich interaction domain 4A, also known as retinoblastoma-binding protein 1, RBBP1/RBP1) is a retinoblastoma (RB)-binding protein. ARID1A is an important subunit of the switching defective/sucrose nonfermenting (SWI/SNF) chromatin remodeling complex.