Validation Data Gallery
|Positive WB detected in||HepG2 cells, HEK-293 cells, NCCIT cell|
|Positive IF detected in||MDCK cells, mouse heart tissue|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66739-1-Ig targets ARL13B in WB, IF, ELISA applications and shows reactivity with Canine, Human, mouse, pig samples.
|Tested Reactivity||Canine, Human, mouse, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||ARL13B fusion protein Ag12031|
|Full Name||ADP-ribosylation factor-like 13B|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC094725|
|Gene ID (NCBI)||200894|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).
INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex
Multiple ciliary localization signals control INPP5E ciliary targeting
Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts.
CCP1, a Tubulin Deglutamylase, Increases Survival of Rodent Spinal Cord Neurons following Glutamate-Induced Excitotoxicity.
Embryonic and foetal expression patterns of the ciliopathy gene CEP164.
Life Sci Alliance
HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lindsey (Verified Customer) (05-24-2021)
I much prefer the Rabbit polyclonal ARL13B (Cat#17711-I-AP) but it is really nice to have an Arl13B Mouse monoclonal for use in co-stains with other targets. I wish it didn't have to be used at such a high working concentration!
Feng-Qian (Verified Customer) (04-04-2019)
This antibody works for detecting primary cilia in MEFs with low background. The intensity is lower than the Arl13B rabbit antibody (17711-1-AP) in general but specific.
Francesc (Verified Customer) (11-07-2018)
This antibody works beautifully, staining cilia with high intensity and specificity, as expected for ARL13B.The staining with 66739-1-Ig appears as good as that obtained with the highly validated Proteintech's rabbit anti-ARL13B (17711-1-AP).This mouse anti-ARL13B makes for a great ciliary marker.