ARL13B Monoclonal antibody

ARL13B Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Canine, Human, mouse, pig and More (2)

Applications

WB, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1H6C3

Cat no : 66739-1-Ig

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, NCCIT cell
Positive IF detected inMDCK cells
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 6 publications below

Product Information

66739-1-Ig targets ARL13B in WB, IF,ELISA applications and shows reactivity with Canine, Human, mouse, pig samples.

Tested Reactivity Canine, Human, mouse, pig
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ARL13B fusion protein Ag12031
Full Name ADP-ribosylation factor-like 13B
Calculated molecular weight 48 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC094725
Gene symbol ARL13B
Gene ID (NCBI) 200894
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Protocols

Product Specific Protocols
WB protocol for ARL13B antibody 66739-1-IgDownload protocol
IF protocol for ARL13B antibody 66739-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Rep

The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium.

Authors - Asma Boukhalfa
humanIF

BMC Nephrol

Use of patient derived urine renal epithelial cells to confirm pathogenicity of PKHD1 alleles.

Authors - Elisa Molinari
mouseIF

Life Sci Alliance

HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails.

Authors - Pablo Barbeito
humanIF

PLoS One

Embryonic and foetal expression patterns of the ciliopathy gene CEP164.

Authors - L A Devlin
ratIF

eNeuro

CCP1, a Tubulin Deglutamylase, Increases Survival of Rodent Spinal Cord Neurons following Glutamate-Induced Excitotoxicity.

Authors - Yasmin H Ramadan
humanIF

Sci Rep

Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts.

Authors - Elisa Molinari

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lindsey (Verified Customer) (05-24-2021)

I much prefer the Rabbit polyclonal ARL13B (Cat#17711-I-AP) but it is really nice to have an Arl13B Mouse monoclonal for use in co-stains with other targets. I wish it didn't have to be used at such a high working concentration!

  • Applications: Immunohistochemistry,Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Human kidney (FFPE)
ARL13B Antibody Immunohistochemistry,Immunofluorescence validation (1:50 dilution) in Human kidney (FFPE) (Cat no:66739-1-Ig)
FH

Feng-Qian (Verified Customer) (04-04-2019)

This antibody works for detecting primary cilia in MEFs with low background. The intensity is lower than the Arl13B rabbit antibody (17711-1-AP) in general but specific.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: methanol fixed Mouse embryonic fibroblasts
FH

Francesc (Verified Customer) (11-07-2018)

This antibody works beautifully, staining cilia with high intensity and specificity, as expected for ARL13B.The staining with 66739-1-Ig appears as good as that obtained with the highly validated Proteintech's rabbit anti-ARL13B (17711-1-AP).This mouse anti-ARL13B makes for a great ciliary marker.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: IMCD3 cells (mouse kidney cell line)
ARL13B Antibody Immunofluorescence, validation (1:100 dilution) in IMCD3 cells (mouse kidney cell line) (Cat no:66739-1-Ig)