ARL13B Monoclonal antibody

ARL13B Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a


Canine, Human, mouse, pig and More (2)







Cat no : 66739-1-Ig


ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, NCCIT cell
Positive IF detected inhTERT-RPE1 cells, mouse heart tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66739-1-Ig targets ARL13B in WB, IF, ELISA applications and shows reactivity with Canine, Human, mouse, pig samples.

Tested Reactivity Canine, Human, mouse, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ARL13B fusion protein Ag12031
Full Name ADP-ribosylation factor-like 13B
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC094725
Gene Symbol ARL13B
Gene ID (NCBI) 200894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).


Product Specific Protocols
WB protocol for ARL13B antibody 66739-1-IgDownload protocol
IF protocol for ARL13B antibody 66739-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex

Authors - Lauren G. Mascibroda


Multiple ciliary localization signals control INPP5E ciliary targeting

Authors - Dario Cilleros-Rodriguez

Sci Rep

Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts.

Authors - Elisa Molinari


CCP1, a Tubulin Deglutamylase, Increases Survival of Rodent Spinal Cord Neurons following Glutamate-Induced Excitotoxicity.

Authors - Yasmin H Ramadan

PLoS One

Embryonic and foetal expression patterns of the ciliopathy gene CEP164.

Authors - L A Devlin

Life Sci Alliance

HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails.

Authors - Pablo Barbeito


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Lindsey (Verified Customer) (05-24-2021)

I much prefer the Rabbit polyclonal ARL13B (Cat#17711-I-AP) but it is really nice to have an Arl13B Mouse monoclonal for use in co-stains with other targets. I wish it didn't have to be used at such a high working concentration!

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Human kidney (FFPE)
ARL13B Antibody Immunohistochemistry,Immunofluorescence validation (1:50 dilution) in Human kidney (FFPE) (Cat no:66739-1-Ig)

Feng-Qian (Verified Customer) (04-04-2019)

This antibody works for detecting primary cilia in MEFs with low background. The intensity is lower than the Arl13B rabbit antibody (17711-1-AP) in general but specific.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: methanol fixed Mouse embryonic fibroblasts

Francesc (Verified Customer) (11-07-2018)

This antibody works beautifully, staining cilia with high intensity and specificity, as expected for ARL13B.The staining with 66739-1-Ig appears as good as that obtained with the highly validated Proteintech's rabbit anti-ARL13B (17711-1-AP).This mouse anti-ARL13B makes for a great ciliary marker.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: IMCD3 cells (mouse kidney cell line)
ARL13B Antibody Immunofluorescence, validation (1:100 dilution) in IMCD3 cells (mouse kidney cell line) (Cat no:66739-1-Ig)