Tested Applications
| Positive WB detected in | HUVEC cells, human brain tissue |
| Positive IP detected in | HUVEC cells |
| Positive IHC detected in | human colon cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10202-1-AP targets ARL4C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0246 Product name: Recombinant human ARL4C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-174 aa of BC001051 Sequence: MLGLDSAGKTTVLYRLKFNEFVNTVPTIGFNTEKIKLSNGTAKGISCHFWDVGGQEKLRPLWKSYSRCTDGIIYVVDSVDVDRLEEAKTELHKVTKFAENQGTPLLVIANKQDLPKSLPVAEIEKQLALHELIPATTYHVQPACAIIGEGLTEGMDKLYEMILKRRKSLKQKKK Predict reactive species |
| Full Name | ADP-ribosylation factor-like 4C |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 21-25 kDa |
| GenBank Accession Number | BC001051 |
| Gene Symbol | ARL4C |
| Gene ID (NCBI) | 10123 |
| RRID | AB_2058661 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56559 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ARL4C antibody 10202-1-AP | Download protocol |
| IHC protocol for ARL4C antibody 10202-1-AP | Download protocol |
| IP protocol for ARL4C antibody 10202-1-AP | Download protocol |
| WB protocol for ARL4C antibody 10202-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARL4C Polyclonal antibody (Cat# 10202-1-AP) has 15 citations. Journals include Cell, Nature Communications, Genome Medicine, PNAS, Journal of Translational Medicine, Frontiers in Pharmacology, Cancer Science, iScience, and others. Research fields span oncology (lung, pancreatic, gastric, colorectal, endometrial, renal, ovarian cancers), neuroscience (hippocampal morphogenesis), osteoarthritis, and epigenomics.
| Species | Application | Title |
|---|---|---|
Cell A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. | ||
Nat Commun TP63 mediates the generation of tumour-specific chromatin loops that underlie MYC activation in radiation-induced tumorigenesis. | ||
Genome Med Integrative epigenomic and high-throughput functional enhancer profiling reveals determinants of enhancer heterogeneity in gastric cancer.
| ||
J Transl Med A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer | ||
Proc Natl Acad Sci U S A CRL5-dependent regulation of the small GTPases ARL4C and ARF6 controls hippocampal morphogenesis.
| ||
Int J Surg Deep learning algorithms from histopathological images stratify molecular subtypes for leiomyosarcoma: a proof-and-concept diagnostic study |
