ARL4C Polyclonal antibody

• Featured Product
• KD/KO Validated

ARL4C Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 10202-1-AP

Synonyms

ARL4C, ARL7, LAK

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Validation Data Gallery

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  WB analysis of Jurkat using 10202-1-AP

  WB result of ARL4C antibody (10202-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARL4C transfected Jurkat cells.

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  WB analysis of HUVEC using 10202-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 10202-1-AP (ARL4C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10202-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10202-1-AP (ARL4C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HUVEC using 10202-1-AP

  IP result of anti-ARL4C (IP:10202-1-AP, 4ug; Detection:10202-1-AP 1:500) with HUVEC cells lysate 3600 ug.

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  IHC staining of human colon cancer using 10202-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10202-1-AP (ARL4C Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10202-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10202-1-AP (ARL4C Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 10202-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10202-1-AP (ARL4C Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 10202-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10202-1-AP (ARL4C Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HUVEC using 10202-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 10202-1-AP (ARL4C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HUVEC cells, Jurkat cells, human brain tissue
• Positive IP detected in: HUVEC cells
• Positive IHC detected in: human colon cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Product Information

10202-1-AP targets ARL4C in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARL4C fusion protein Ag0246
• Full Name: ADP-ribosylation factor-like 4C
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21-25 kDa
• GenBank Accession Number: BC001051
• Gene Symbol: ARL4C
• Gene ID (NCBI): 10123
• RRID: AB_2058661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Cell; A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.; Authors - Banaszynski Laura A LA
human	WB	Genome Med; Integrative epigenomic and high-throughput functional enhancer profiling reveals determinants of enhancer heterogeneity in gastric cancer.; Authors - Taotao Sheng ; KD Validated
mouse	WB, IF	Proc Natl Acad Sci U S A; CRL5-dependent regulation of the small GTPases ARL4C and ARF6 controls hippocampal morphogenesis.; Authors - Jisoo S Han ; KD Validated
human	WB,IHC	Cancer Sci; ARL4C is associated with initiation and progression of lung adenocarcinoma and represents a therapeutic target.; Authors - Kenji Kimura
human	WB,IF	iScience; Arl4c promotes the growth and drug resistance of pancreatic cancer by regulating tumor-stromal interactions.; Authors - Xin Chen ; KD Validated
human	IHC	Cancer Biomark; ADP-ribosylation factor-like 4C predicts worse prognosis in endometriosis-associated ovarian cancers; Authors - Shiro Wakinoue