ARMET Recombinant monoclonal antibody

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  WB analysis using 86360-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86360-2-RR (ARMET antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86360-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86360-2-RR (ARMET antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86360-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86360-2-RR against Human ARMET were performed. The affinity constant is below 1 pM.

ARMET Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86360-2-RR

Clone No.250463C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Arginine-rich protein, ARP, MANF, Mesencephalic astrocyte-derived neurotrophic factor

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Tested Applications

• Positive WB detected in: HT-29 cells, HepG2 cells, mouse pancreas tissue, rat pancreas tissue, BxPC-3 cells, mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86360-2-RR targets ARMET in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1115

  Product name: Recombinant human ARMET protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-182 aa of BC007282

  Sequence: MRRMWATQGLAVALALSVLPGSRALRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL

• Full Name: arginine-rich, mutated in early stage tumors
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 13-20 kDa
• GenBank Accession Number: BC007282
• Gene Symbol: ARMET
• Gene ID (NCBI): 7873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P55145
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.