ARMET Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 86360-2-RR (same clone as 86360-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86360-2-RR (ARMET antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86360-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 86360-2-RR (same clone as 86360-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86360-2-RR (ARMET antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86360-2-PBS in a different storage buffer formulation.

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  Sample test of MP02377-1

  The mean ARMET concentration was determined to be 4.1 ng/mL in HuH-7 cell extract based on a 1.4 mg/mL extract load.

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  Cytometric bead array standard curve of MP02377-1

  Cytometric bead array standard curve of MP02377-1, ARMET Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86360-2-PBS. Detection antibody: 86360-1-PBS. Standard: Ag1115. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 86360-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86360-2-RR against Human ARMET were performed. The affinity constant is below 1 pM.

ARMET Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 86360-2-PBS

Clone No.250463C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

Arginine-rich protein, ARP, MANF, Mesencephalic astrocyte-derived neurotrophic factor

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Product Information

86360-2-PBS targets ARMET as part of a matched antibody pair:

MP02377-1: 86360-2-PBS capture and 86360-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1115

  Product name: Recombinant human ARMET protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-182 aa of BC007282

  Sequence: MRRMWATQGLAVALALSVLPGSRALRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL

• Full Name: arginine-rich, mutated in early stage tumors
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 13-20 kDa
• GenBank Accession Number: BC007282
• Gene Symbol: ARMET
• Gene ID (NCBI): 7873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P55145
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.