ARNT,HIF1B Monoclonal antibody

ARNT,HIF1B Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3B12C5

Cat no : 66732-1-Ig

Synonyms

ARNT, ARNT protein, ARNT,HIF1B, bHLHe2, HIF 1 beta, HIF 1beta, HIF1 beta, HIF1B, HIF1BETA, TANGO

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Validation Data Gallery

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  WB analysis using 66732-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66732-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66732-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 66732-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66732-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ARNT,HIF1B (66732-1-Ig, Clone:3B12C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HeLa using 66732-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ARNT,HIF1B (66732-1-Ig, Clone:3B12C5) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: Caco-2 cells, HeLa cells, NIH/3T3 cells, HepG2 cells, HT-29 cells, HSC-T6 cells
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

66732-1-Ig targets ARNT,HIF1B in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARNT,HIF1B fusion protein Ag5507
• Full Name: aryl hydrocarbon receptor nuclear translocator
• Calculated molecular weight: 416 aa, 46 kDa, 87 kDa
• Observed molecular weight: 87 kDa
• GenBank accession number: BC060838
• Gene symbol: ARNT
• Gene ID (NCBI): 405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia