ARNT,HIF1B Monoclonal antibody

ARNT,HIF1B Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, rat







Cat no : 66732-1-Ig


ARNT, ARNT protein, ARNT,HIF1B, bHLHe2, HIF 1 beta, HIF 1beta, HIF1 beta, HIF1B, HIF1BETA, TANGO

Tested Applications

Positive WB detected inCaco-2 cells, HeLa cells, HepG2 cells, HT-29 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66732-1-Ig targets ARNT,HIF1B in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARNT,HIF1B fusion protein Ag5507
Full Name aryl hydrocarbon receptor nuclear translocator
Calculated Molecular Weight 416 aa, 46 kDa, 87 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC060838
Gene Symbol ARNT
Gene ID (NCBI) 405
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia


Product Specific Protocols
WB protocol for ARNT,HIF1B antibody 66732-1-IgDownload protocol
IF protocol for ARNT,HIF1B antibody 66732-1-IgDownload protocol
Standard Protocols
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