ARNT,HIF1B Monoclonal antibody
ARNT,HIF1B Monoclonal Antibody for FC, IF, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
WB, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
3B12C5
Cat no : 66732-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Caco-2 cells, HeLa cells, NIH/3T3 cells, HepG2 cells, HT-29 cells, HSC-T6 cells |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
66732-1-Ig targets ARNT,HIF1B in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | ARNT,HIF1B fusion protein Ag5507 |
Full Name | aryl hydrocarbon receptor nuclear translocator |
Calculated molecular weight | 416 aa, 46 kDa, 87 kDa |
Observed molecular weight | 87 kDa |
GenBank accession number | BC060838 |
Gene symbol | ARNT |
Gene ID (NCBI) | 405 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia
Protocols
Product Specific Protocols | |
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WB protocol for ARNT,HIF1B antibody 66732-1-Ig | Download protocol |
IF protocol for ARNT,HIF1B antibody 66732-1-Ig | Download protocol |
FC protocol for ARNT,HIF1B antibody 66732-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |