CoraLite®594-conjugated ARNT,HIF1B Monoclonal antibody

ARNT,HIF1B Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3B12C5

Cat no : CL594-66732

Synonyms

ARNT, ARNT protein, ARNT,HIF1B, bHLHe2, HIF 1 beta, HIF 1beta, HIF1 beta, HIF1B, HIF1BETA, TANGO



Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66732-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66732 targets ARNT,HIF1B in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARNT,HIF1B fusion protein Ag5507
Full Name aryl hydrocarbon receptor nuclear translocator
Calculated molecular weight 416 aa, 46 kDa, 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC060838
Gene symbol ARNT
Gene ID (NCBI) 405
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

Protocols

Product Specific Protocols
IF protocol for CL594 ARNT,HIF1B antibody CL594-66732Download protocol
FC protocol for CL594 ARNT,HIF1B antibody CL594-66732Download protocol
Standard Protocols
Click here to view our Standard Protocols