CoraLite® Plus 488-conjugated ARNT/HIF-1 beta Recombinant monoclonal antibody

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  IF Staining of HepG2 using CL488-84650-4

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ARNT/HIF-1 beta antibody (CL488-84650-4, Clone: 242111G2 ) at dilution of 1:200, CL594-Phalloidin (red).

ARNT/HIF-1 beta Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-84650-4

Clone No.242111G2

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

Synonyms

ARNT, HIF-1 beta, HIF1B, 242111G2, bHLHe2

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84650-4 targets ARNT/HIF-1 beta in applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5507

  Product name: Recombinant human ARNT,HIF1B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-348 aa of BC060838

  Sequence: MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQQQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSS

• Full Name: aryl hydrocarbon receptor nuclear translocator
• Calculated Molecular Weight: 416 aa, 46 kDa, 87 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC060838
• Gene Symbol: ARNT/HIF-1 beta
• Gene ID (NCBI): 405
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P27540
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.