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ARPC1B Polyclonal antibody

ARPC1B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28368-1-AP

Synonyms

ARC41, Arp2/3 complex 41 kDa subunit, ARPC1B, p40 ARC, p41 ARC



Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

28368-1-AP targets ARPC1B in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARPC1B fusion protein Ag29001
Full Name actin related protein 2/3 complex, subunit 1B, 41kDa
Calculated molecular weight 41 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC007555
Gene symbol ARPC1B
Gene ID (NCBI) 10095
RRIDAB_2881125
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARPC1B also known as ARC41, p41-ARC, or p40-ARC, is one of seven subunits of the human Arp2/3 protein complex, that belongs to the SOP2 family. ARPC1B localizes on centrosomes and has a distinct role in centrosomal homeostasis. ARPC1B is both a physiological activator and substrate of Aurora A kinase, and these interactions help to maintain mitotic integrity in mammalian cells. In recent resaeach, ARPC1B is selected as a candidate prediction marker for sensitivity of Choroidal malignant melanomas to radiotherapy.

Protocols

Product Specific Protocols
WB protocol for ARPC1B antibody 28368-1-APDownload protocol
IHC protocol for ARPC1B antibody 28368-1-APDownload protocol
IP protocol for ARPC1B antibody 28368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Front Immunol

The overexpression of actin related protein 2/3 complex subunit 1B(ARPC1B) promotes the ovarian cancer progression via activation of the Wnt/β-catenin signaling pathway

Authors - Junning Huang