ARPC2 Monoclonal antibody, PBS Only

ARPC2 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D8E3

Cat no : 68145-1-PBS

Synonyms

ARC34, Arp2/3 complex 34 kDa subunit, ARPC2, p34 Arc, PNAS 139, PRO2446

---

Validation Data Gallery

• 

  WB analysis using 68145-1-Ig (same clone as 68145-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68145-1-Ig (ARPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 68145-1-Ig (same clone as 68145-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 68145-1-Ig (same clone as 68145-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68145-1-Ig (same clone as 68145-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ARPC2 antibody (68145-1-Ig, Clone: 1D8E3 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 68145-1-Ig (same clone as 68145-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (68145-1-Ig, Clone:1D8E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68145-1-PBS targets ARPC2 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARPC2 fusion protein Ag7082
• Full Name: actin related protein 2/3 complex, subunit 2, 34kDa
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000590
• Gene Symbol: ARPC2
• Gene ID (NCBI): 10109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.