ARPC2 Monoclonal antibody

ARPC2 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1D8E3

Cat no : 68145-1-Ig

Synonyms

ARC34, Arp2/3 complex 34 kDa subunit, ARPC2, p34 Arc, PNAS 139, PRO2446



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, human peripheral blood platelets, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

68145-1-Ig targets ARPC2 in WB, IHC, FC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARPC2 fusion protein Ag7082
Full Name actin related protein 2/3 complex, subunit 2, 34kDa
Calculated molecular weight 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC000590
Gene symbol ARPC2
Gene ID (NCBI) 10109
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARPC2 antibody 68145-1-IgDownload protocol
IHC protocol for ARPC2 antibody 68145-1-IgDownload protocol
FC protocol for ARPC2 antibody 68145-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols