ARSF Polyclonal antibody

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  WB analysis of COS-7 using 16561-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16561-1-AP (ARSF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ARSF Polyclonal Antibody for WB, ELISA

Cat No. 16561-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Monkey

Applications

WB, ELISA

Synonyms

ARSF, arylsulfatase F, ASF

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Tested Applications

• Positive WB detected in: COS-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16561-1-AP targets ARSF in WB, ELISA applications and shows reactivity with Monkey samples.

• Tested Reactivity: Monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9790

  Product name: Recombinant human ARSF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 233-590 aa of BC022389

  Sequence: WFSSHTSPLYWDCLLMRGHEITEQPMKAERAGSIMVKEAISFLERHSKETFLLFFSFLHVHTPLPTTDDFTGTSKHGLYGDNVEEMDSMVGKILDAIDDFGLRNNTLVYFTSDHGGHLEARRGHAQLGGWNGIYKGGKGMGGWEGGIRVPGIVRWPGKVPAGRLIKEPTSLMDILPTVASVSGGSLPQDRVIDGRDLMPLLQGNVRHSEHEFLFHYCGSYLHAVRWIPKDDSGSVWKAHYVTPVFQPPASGGCYVTSLCRCFGEQVTYHNPPLLFDLSRDPSESTPLTPATEPLYDFVIKKVANALKEHQETIVPVTYQLSELNQGRTWLKPCCGVFPFCLCDKEEEVSQPRGPNEKR

• Full Name: arylsulfatase F
• Calculated Molecular Weight: 590 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC022389
• Gene Symbol: ARSF
• Gene ID (NCBI): 416
• RRID: AB_3085497
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P54793
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Arylsulfatase F (ARSF) is located on the X-chromosome and belongs to the sulfatase family. ARSF exhibits arylsulfatase activity towards the artificial substrate 4-methylumbelliferyl sulfate. ARSF expression is enhanced in the brain, kidney, and skin. The molecular weight of ARSF is 66 kDa.