ART1 Monoclonal antibody

ART1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1D10A8

Cat no : 66958-1-Ig

Synonyms

ADP ribosyltransferase 1, ART1, ART2, CD296, Mono(ADP ribosyl)transferase 1, RT6

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Validation Data Gallery

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  WB analysis using 66958-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66958-1-Ig (ART1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66958-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66958-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66958-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66958-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: EC109 cells, HeLa cells, HEK-293 cells, HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

66958-1-Ig targets ART1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ART1 fusion protein Ag28269
• Full Name: ADP-ribosyltransferase 1
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34-36 kDa
• GenBank Accession Number: NM_004314
• Gene Symbol: ART1
• Gene ID (NCBI): 417
• RRID: AB_2882281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ART1 (ADP-ribosyltransferase 1) is an important mono-ADP-ribose transferase which can catalyze the mono-ADP-ribosylation of proteins including human neutrophil peptide-1 (HNP-1), fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor-BB (PDGF-BB), and play a role in biological processes such as proliferation and invasion of cancer cells.

Publications

Species	Application	Title
mouse	WB	Med Oncol; Identification of the role of mono-ADP-ribosylation in colorectal cancer by integrated transcriptome analysis.; Authors - Shuxian Zhang
human,mouse	IHC,IF,WB	BMC Cancer; ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells; Authors - Ting Lin ; KD Validated