Tested Applications
| Positive WB detected in | pig liver tissue, pig brain tissue, rabbit brain tissue, rat brain tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67092-2-Ig targets ASAH1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig, rabbit samples.
| Tested Reactivity | human, rat, pig, rabbit |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28645 Product name: Recombinant human ASAH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-389 aa of BC016828 Sequence: MNCCIGLGEKARGSHRASYPSLSALFTEASILGFGSFAVKAQWTEDCRKSTYPPSGPTVFPAVIRYRGAVPWYTINLDLPPYKRWHELMLDKAPVPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTVCTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDAMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW Predict reactive species |
| Full Name | N-acylsphingosine amidohydrolase (acid ceramidase) 1 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44-55 kDa |
| GenBank Accession Number | BC016828 |
| Gene Symbol | ASAH1 |
| Gene ID (NCBI) | 427 |
| RRID | AB_3085035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13510 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ASAH1 antibody 67092-2-Ig | Download protocol |
| WB protocol for ASAH1 antibody 67092-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ASAH1 Monoclonal antibody (2B3C7), catalog number 67092-2-Ig, has 1 total citation. The cited publication appeared in Advanced Science (Impact Factor 14.1), published in 2026. The research field is cancer biology, specifically investigating how disrupting lipid raft microdomains blocks polyploid giant cancer cell budding and enhances radiotherapy response. The antibody was used in Western blot and immunofluorescence applications on human samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Disrupting Lipid Raft Microdomains to Block Polyploid Giant Cancer Cell Budding and Enhance Radiotherapy Response. |
