ASC/TMS1 Monoclonal antibody

ASC/TMS1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2G12E6

Cat no : 67494-1-Ig

Synonyms

ASC, ASC/TMS1, CARD5, hASC, PYCARD, PYD and CARD domain containing, TMS, TMS 1, TMS1



Tested Applications

Positive WB detected inHL-60 cells, human placenta tissue, rat spleen tissue, THP-1 cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

67494-1-Ig targets ASC/TMS1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASC/TMS1 fusion protein Ag28424
Full Name PYD and CARD domain containing
Calculated molecular weight 25 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC004470
Gene symbol PYCARD
Gene ID (NCBI) 29108
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

Protocols

Product Specific Protocols
WB protocol for ASC/TMS1 antibody 67494-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
mouseWB

Oxid Med Cell Longev

PPARγ Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS/TXNIP/NLRP3 Signaling Pathway.

Authors - Zeyu Li
mouseWB

J Neuroinflammation

Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.

Authors - Shiji Huo
mouseWB,IF

J Virol

Pseudorabies Virus Infection Activates the TLR-NF-κB Axis and AIM2 Inflammasome To Enhance Inflammatory Responses in Mice

Authors - Qiongqiong Zhou
mouseWB

Clin Sci (Lond)

Buformin alleviates sepsis-induced acute lung injury via inhibiting NLRP3-mediated pyroptosis through an AMPK-dependent pathway.

Authors - Bohao Liu
mouseWB

Front Microbiol

Trichodimerol inhibits inflammation through suppression of the nuclear transcription factor-kappaB/NOD-like receptor thermal protein domain associated protein 3 signaling pathway

Authors - Xue-Yan Huo