ASC/TMS1 Monoclonal antibody

ASC/TMS1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G12E6

Cat no : 67494-1-Ig

Synonyms

ASC, ASC/TMS1, CARD5, hASC, PYCARD, PYD and CARD domain containing, TMS, TMS 1, TMS1



Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

67494-1-Ig targets ASC/TMS1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASC/TMS1 fusion protein Ag28424
Full Name PYD and CARD domain containing
Calculated molecular weight 25 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC004470
Gene symbol PYCARD
Gene ID (NCBI) 29108
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

Protocols

Product Specific Protocols
WB protocol for ASC/TMS1 antibody 67494-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.

Authors - Shiji Huo
mouseWB

Clin Sci (Lond)

Buformin alleviates sepsis-induced acute lung injury via inhibiting NLRP3-mediated pyroptosis through an AMPK-dependent pathway.

Authors - Bohao Liu
mouseWB

Front Microbiol

Trichodimerol inhibits inflammation through suppression of the nuclear transcription factor-kappaB/NOD-like receptor thermal protein domain associated protein 3 signaling pathway

Authors - Xue-Yan Huo
mouseWB

Cardiovasc Drugs Ther

Disulfiram protects against abdominal aortic aneurysm by ameliorating vascular smooth muscle cells pyroptosis.

Authors - Fei Liao
mouseWB

Sci Rep

Aronia melanocarpa polysaccharide ameliorates inflammation and aging in mice by modulating the AMPK/SIRT1/NF-κB signaling pathway and gut microbiota

Authors - Yingchun Zhao
mouseWB,IHC

Int Immunopharmacol

Curcumin analogue AI-44 alleviates MSU-induced gouty arthritis in mice via inhibiting cathepsin B-mediated NLRP3 inflammasome activation.

Authors - Yanpin Gu