Tested Applications
| Positive WB detected in | HL-60 cells, human placenta tissue, THP-1 cells, U-937 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, THP-1 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67494-1-Ig targets ASC/TMS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28424 Product name: Recombinant human TMS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-135 aa of BC004470 Sequence: MGRARDAILDALENLTAEELKKFKLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS Predict reactive species |
| Full Name | PYD and CARD domain containing |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 22-25 kDa |
| GenBank Accession Number | BC004470 |
| Gene Symbol | ASC/TMS1 |
| Gene ID (NCBI) | 29108 |
| RRID | AB_2882718 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9ULZ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ASC/TMS1 antibody 67494-1-Ig | Download protocol |
| IHC protocol for ASC/TMS1 antibody 67494-1-Ig | Download protocol |
| WB protocol for ASC/TMS1 antibody 67494-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ASC/TMS1 Monoclonal antibody (2G12E6), catalog number 67494-1-Ig, has 62 total citations published across 48 journals, including Molecular Cell, Science Advances, Cell Reports, eBioMedicine, Cell Death & Discovery, and EMBO Reports. Associated research fields span inflammasome biology (NLRP3), pyroptosis, PANoptosis, cancer, neuroinflammation, cardiovascular disease, liver fibrosis, metabolic disorders, autoimmune diseases, and infectious disease.
| Species | Application | Title |
|---|---|---|
Drug Resist Updat Targeting LIG1 to overcome oxaliplatin resistance by inducing PANoptosis in colorectal cancer. | ||
Exp Hematol Oncol Tetrahydromagnolol targets TRIM38 to mediate PANoptosis in cancer cells and has the potential for synergistic cancer therapy. | ||
Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation | ||
Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation | ||
Sci Adv Asparagine transporter supports macrophage inflammation via histone phosphorylation | ||
J Neuroinflammation Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice. |
