ASC/TMS1 Monoclonal antibody

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  WB analysis using 67494-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of human placenta using 67494-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 67494-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

ASC/TMS1 Monoclonal Antibody for WB, ELISA

Cat No. 67494-1-Ig

Clone No.2G12E6

Publications(31)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

PYCARD, hASC, Caspase recruitment domain-containing protein 5, CARD5, ASC

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Tested Applications

• Positive WB detected in: HL-60 cells, human placenta tissue, THP-1 cells, U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 25 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

67494-1-Ig targets ASC/TMS1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASC/TMS1 fusion protein Ag28424
• Full Name: PYD and CARD domain containing
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC004470
• Gene Symbol: ASC/TMS1
• Gene ID (NCBI): 29108
• RRID: AB_2882718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9ULZ3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

Publications

Species	Application	Title
	IF	Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
	WB,IF,IP	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
	WB	Oxid Med Cell Longev; PPARγ Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS/TXNIP/NLRP3 Signaling Pathway.; Authors - Zeyu Li
	WB	J Neuroinflammation; Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.; Authors - Shiji Huo
	WB,IF	J Virol; Pseudorabies Virus Infection Activates the TLR-NF-κB Axis and AIM2 Inflammasome To Enhance Inflammatory Responses in Mice; Authors - Qiongqiong Zhou
	WB	J Ethnopharmacol; Xiaoqinglong decoction improves allergic rhinitis by inhibiting NLRP3-mediated pyroptosis in BALB/C mice; Authors - Ruizhi Wang