ATAD1 Polyclonal antibody

ATAD1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16882-1-AP

Synonyms

AFDC1, ATAD1, FNP001

---

Validation Data Gallery

• 

  WB analysis using 16882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16882-1-AP (ATAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16882-1-AP

  IP result of anti-ATAD1 (IP:16882-1-AP, 4ug; Detection:16882-1-AP 1:1000) with mouse brain tissue lysate 1280 ug.

---

Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16882-1-AP targets ATAD1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATAD1 fusion protein Ag10343
• Full Name: ATPase family, AAA domain containing 1
• Calculated Molecular Weight: 361 aa, 41 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC010868
• Gene Symbol: ATAD1
• Gene ID (NCBI): 84896
• RRID: AB_2878327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.