ATF1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 87421-1-RR (same clone as 87421-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87421-1-RR (ATF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87421-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 87421-1-RR (same clone as 87421-1-PBS)

  WB result of ATF1 antibody (87421-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF1 transfected A549 cells. This data was developed using the same antibody clone with 87421-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 87421-1-RR (same clone as 87421-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF1 antibody (87421-1-RR, Clone: 252816B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87421-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 87421-1-RR (same clone as 87421-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ATF1 antibody (87421-1-RR, Clone: 252816B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87421-1-PBS in a different storage buffer formulation.

ATF1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87421-1-PBS

Clone No.252816B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

cAMP-dependent transcription factor ATF-1, Cyclic AMP-dependent transcription factor ATF-1, TREB36

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Product Information

87421-1-PBS targets ATF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2548

  Product name: Recombinant human ATF1 protein

  Source: e coli.-derived, T-GST

  Tag: GST

  Domain: 1-271 aa of BC029619

  Sequence: MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV

• Full Name: activating transcription factor 1
• Calculated Molecular Weight: 271 aa, 29 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC029619
• Gene Symbol: ATF1
• Gene ID (NCBI): 466
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P18846
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TFs play a vital role in maintaining cell homeostasis by combining DNA regulatory sequences to regulate gene transcription rates. Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site 'TGACGTCA'(PMID: 28032861). ATF1 transcriptionally regulates the expressions of target genes to mediate various cellular processes, and ATF1 effects the progression of tumors (including lung cancer, colorectal cancer,and nasopharyngeal carcinoma).