ATG16L1 Monoclonal antibody

ATG16L1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1G5G7

Cat no : 67943-1-Ig

Synonyms

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30

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Validation Data Gallery

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  WB analysis using 67943-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of rat lung using 67943-1-Ig

  rat lung tissue were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67943-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67943-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG16L1 antibody (67943-1-Ig, Clone: 1G5G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HSC-T6 cells, HepG2 cells, rat lung tissue, PC-12 cells, NIH/3T3 cells, 4T1 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

67943-1-Ig targets ATG16L1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATG16L1 fusion protein Ag14881
• Full Name: ATG16 autophagy related 16-like 1 (S. cerevisiae)
• Calculated Molecular Weight: 607 aa, 68 kDa
• Observed Molecular Weight: 68-75 kDa
• GenBank Accession Number: BC000061
• Gene Symbol: ATG16L1
• Gene ID (NCBI): 55054
• RRID: AB_2918695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)

Publications

Species	Application	Title
pig	WB	Front Immunol; Porcine cGAS-STING signaling induced autophagy inhibits STING downstream IFN and apoptosis; Authors - Nengwen Xia
human	WB	Epileptic Disord; lncRNA UCA1 induces autophagic gene expression via epigenetic regulation mediated by ATG16L1 and miR-132-3p in SH-SY5Y cells treated with retinoic acid; Authors - Fang Wen
human	WB	Mol Carcinog; Forkhead Box Q1 is a novel regulator of autophagy in breast cancer cells; Authors - Su-Hyeong Kim
human	WB,IF	Dev Cell; V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation; Authors - Yiwei Sun