ATG2A Polyclonal antibody

ATG2A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23226-1-AP

Synonyms

ATG2A, KIAA0404



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23226-1-AP is ATG2A Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG2A fusion protein Ag19709
Full Name ATG2 autophagy related 2 homolog A (S. cerevisiae)
Calculated molecular weight 1938 aa, 213 kDa
Observed molecular weight 215 kDa
GenBank accession numberBC110650
Gene symbol ATG2A
Gene ID (NCBI) 23130
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATG2A antibody 23226-1-APDownload protocol
IHC protocol for ATG2A antibody 23226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.

Authors - Wu Sun
humanWB,IF

EMBO Rep

A conserved ATG2-GABARAP family interaction is critical for phagophore formation.

Authors - Mihaela Bozic