ATG2A Polyclonal antibody

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  WB analysis using 23226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23226-1-AP (ATG2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 23226-1-AP

  IP result of anti-ATG2A (IP:23226-1-AP, 4ug; Detection:23226-1-AP 1:4000) with K-562 cells lysate 2400 ug.

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  IHC staining of mouse kidney using 23226-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23226-1-AP (ATG2A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 23226-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23226-1-AP (ATG2A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 23226-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ATG2A antibody (23226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

ATG2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 23226-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Peptide array

Synonyms

KIAA0404, Autophagy-related protein 2 homolog A

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells, Raji cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

23226-1-AP targets ATG2A in WB, IHC, IF/ICC, IP, CoIP, ELISA, Peptide array applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG2A fusion protein Ag19709
• Full Name: ATG2 autophagy related 2 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 1938 aa, 213 kDa
• Observed Molecular Weight: 215 kDa
• GenBank Accession Number: BC110650
• Gene Symbol: ATG2A
• Gene ID (NCBI): 23130
• RRID: AB_2879235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q2TAZ0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Autophagy-related protein 2 homolog A (ATG2A) belongs to the ATG2 family. ATG2A is a lipid transfer protein involved in autophagosome assembly (PMID:31271352). ATG2A tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion. ATG2A binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PMID: 30952800, PMID: 31271352). ATG2A also regulates lipid droplet morphology and distribution within the cell (PMID: 28561066). The observed MW of ATG2A is 215 kDa.

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB	Nat Cell Biol; ATG9A protects the plasma membrane from programmed and incidental permeabilization.; Authors - Aurore Claude-Taupin ; KO Validated
human	WB,CoIP,Peptide array	Mol Cell; ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation; Authors - Alexander R van Vliet
human	IF	Nat Commun; The autophagy protein ATG9A enables lipid mobilization from lipid droplets.; Authors - Elodie Mailler ; KO Validated
human	WB,IF	EMBO Rep; A conserved ATG2-GABARAP family interaction is critical for phagophore formation.; Authors - Mihaela Bozic
human	WB	Theranostics; The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.; Authors - Wu Sun