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- KD/KO Validated
ATG2A Polyclonal antibody
ATG2A Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, Peptide array, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 23226-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, MCF-7 cells, Raji cells |
Positive IHC detected in | human tonsillitis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 8 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
CoIP | See 1 publications below |
Product Information
23226-1-AP targets ATG2A in WB, Peptide array, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ATG2A fusion protein Ag19709 |
Full Name | ATG2 autophagy related 2 homolog A (S. cerevisiae) |
Calculated molecular weight | 1938 aa, 213 kDa |
Observed molecular weight | 215 kDa |
GenBank accession number | BC110650 |
Gene symbol | ATG2A |
Gene ID (NCBI) | 23130 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for ATG2A antibody 23226-1-AP | Download protocol |
IHC protocol for ATG2A antibody 23226-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol ATG9A protects the plasma membrane from programmed and incidental permeabilization.
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Mol Cell ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation | ||
Nat Commun The autophagy protein ATG9A enables lipid mobilization from lipid droplets.
| ||
EMBO Rep A conserved ATG2-GABARAP family interaction is critical for phagophore formation. | ||
Theranostics The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer. |