ATG2A Polyclonal antibody

• Featured Product
• KD/KO Validated

ATG2A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Peptide array, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 23226-1-AP

Synonyms

ATG2A, KIAA0404

---

Validation Data Gallery

• 

  WB analysis using 23226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23226-1-AP (ATG2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 23226-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23226-1-AP (ATG2A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 23226-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23226-1-AP (ATG2A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 23226-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23226-1-AP (ATG2A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 23226-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23226-1-AP (ATG2A Antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells, Raji cells
• Positive IHC detected in: human tonsillitis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

23226-1-AP targets ATG2A in WB, Peptide array, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG2A fusion protein Ag19709
• Full Name: ATG2 autophagy related 2 homolog A (S. cerevisiae)
• Calculated molecular weight: 1938 aa, 213 kDa
• Observed molecular weight: 215 kDa
• GenBank accession number: BC110650
• Gene symbol: ATG2A
• Gene ID (NCBI): 23130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB	Nat Cell Biol; ATG9A protects the plasma membrane from programmed and incidental permeabilization.; Authors - Aurore Claude-Taupin ; KO Validated
human	WB,CoIP,Peptide array	Mol Cell; ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation; Authors - Alexander R van Vliet
human	IF	Nat Commun; The autophagy protein ATG9A enables lipid mobilization from lipid droplets.; Authors - Elodie Mailler ; KO Validated
human	WB,IF	EMBO Rep; A conserved ATG2-GABARAP family interaction is critical for phagophore formation.; Authors - Mihaela Bozic
human	WB	Theranostics; The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.; Authors - Wu Sun