ATG4C Polyclonal antibody

ATG4C Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20382-1-AP

Synonyms

APG4 C, APG4C, ATG4C, AUTL1, AUTL3, Autophagin 3, Cysteine protease ATG4C



Tested Applications

Positive WB detected inmouse testis tissue, Jurkat cells, mouse brain tissue
Positive IHC detected inhuman heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20382-1-AP is ATG4C Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG4C fusion protein Ag14207
Full Name ATG4 autophagy related 4 homolog C (S. cerevisiae)
Calculated molecular weight 458 aa, 52 kDa
Observed molecular weight 52-55 kDa
GenBank accession numberBC033024
Gene symbol ATG4C
Gene ID (NCBI) 84938
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATG4C antibody 20382-1-APDownload protocol
IHC protocol for ATG4C antibody 20382-1-APDownload protocol
IF protocol for ATG4C antibody 20382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.

Authors - Wu Sun