ATG4C Polyclonal antibody

ATG4C Polyclonal Antibody for WB, IF, IHC, ELISA

Cat No. 20382-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Synonyms

APG4 C, APG4C, ATG4C, AUTL1, AUTL3, Autophagin 3, Cysteine protease ATG4C

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

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WB analysis of Jurkat using 20382-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 20382-1-AP (ATG4C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 20382-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 20382-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 20382-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 20382-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 20382-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATG4C antibody (20382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells
Positive IHC detected in	human heart tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20382-1-AP targets ATG4C in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14207 Product name: Recombinant human ATG4C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC033024 Sequence: MEATGTDEVDKLKTKFISAWNNMKYSWVLKTKTYFSRNSPVLLLGKCYHFKYEDEDKTLPAESGCTIEDHVIAGNVEEFRKDFISRIWLTYREEFPQIEGSALTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALNIENSDSESWTSHTVKKFTASFEASLSGEREFKTPTISLKETIGKYSDDHEMRNEVYHRKIISWFGDSPLALFGLHQLIEYGKKSGKKAGDWYGPAVVAHILRKAVEEARHPDLQGITIYVAQDCTVYNSDVIDKQSASMTSDNADDKAVIILVPVRLGGE Predict reactive species
Full Name	ATG4 autophagy related 4 homolog C (S. cerevisiae)
Calculated Molecular Weight	458 aa, 52 kDa
Observed Molecular Weight	52-55 kDa
GenBank Accession Number	BC033024
Gene Symbol	ATG4C
Gene ID (NCBI)	84938
RRID	AB_10666852
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96DT6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The ATG4C Polyclonal antibody (Cat# 20382-1-AP) has 2 citations. One appeared in Autophagy (IF 18.6), studying ATG4C function in vivo in mouse using WB. The other was published in Theranostics (IF 14.9), investigating the c-Myc/miR-27b-3p/ATG10 regulatory axis in colorectal cancer chemoresistance in human samples via WB. The associated research fields are autophagy and oncology (colorectal cancer).

Species	Application	Title
mouse	WB	Autophagy Analysis of ATG4C function in vivo Authors - Isaac Tamargo-Gómez View Article
human	WB	Theranostics The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer. Authors - Wu Sun View Article