ATG4C Recombinant monoclonal antibody

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  WB analysis using 84146-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84146-1-RR (ATG4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84146-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84146-1-RR against Human ATG4C were performed. The affinity constant is 1.26 nM.

ATG4C Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84146-1-RR

Clone No.230359A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

AUTL3, AUTL1, ATG, APG4C, APG4 C

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84146-1-RR targets ATG4C in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag14207

  Product name: Recombinant human ATG4C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC033024

  Sequence: MEATGTDEVDKLKTKFISAWNNMKYSWVLKTKTYFSRNSPVLLLGKCYHFKYEDEDKTLPAESGCTIEDHVIAGNVEEFRKDFISRIWLTYREEFPQIEGSALTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALNIENSDSESWTSHTVKKFTASFEASLSGEREFKTPTISLKETIGKYSDDHEMRNEVYHRKIISWFGDSPLALFGLHQLIEYGKKSGKKAGDWYGPAVVAHILRKAVEEARHPDLQGITIYVAQDCTVYNSDVIDKQSASMTSDNADDKAVIILVPVRLGGE

• Full Name: ATG4 autophagy related 4 homolog C (S. cerevisiae)
• Calculated Molecular Weight: 458 aa, 52 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: BC033024
• Gene Symbol: ATG4C
• Gene ID (NCBI): 84938
• RRID: AB_3671709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96DT6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Autophagy related 4C cysteine peptidase (ATG4C) is an autophagy regulator responsible for cleaving of pro-LC3 and delipidation of LC3 II.