ATL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 16921-1-AP

Synonyms

EC:3.6.5.-, Atlastin-3, Atlastin 3

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Validation Data Gallery

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  WB analysis using 16921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16921-1-AP

  WB result of ATL3 antibody (16921-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATL3 transfected HeLa cells.

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  WB analysis using 16921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16921-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 16921-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16921-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16921-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16921-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16921-1-AP

  IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:5000) with HeLa cells lysate 1720 ug.

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  IP experiment of HeLa using 16921-1-AP

  IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:8000) with HeLa cells lysate 1760 ug.

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  IHC staining of human stomach cancer using 16921-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16921-1-AP (ATL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16921-1-AP

  Immunofluorescent analysis of HepG2 cells, using ATL3 antibody 16921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 16921-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (16921-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse liver tissue, SMMC-7721 cells, Jurkat cells, NIH/3T3 cells, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

16921-1-AP targets ATL3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATL3 fusion protein Ag10279
• Full Name: atlastin GTPase 3
• Calculated Molecular Weight: 541 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC077727
• Gene Symbol: ATL3
• Gene ID (NCBI): 25923
• RRID: AB_2290228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.

Publications

Species	Application	Title
human	WB,IP	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
human,mouse	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
	WB	Genes Dev; Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein; Authors - Megan L Norris
human	WB,IF	Elife; Efficient protein targeting to the inner nuclear membrane requires Atlastin-dependent maintenance of ER topology.; Authors - Sumit Pawar ; KD Validated
human,monkey	CoIP,WB	Curr Biol; ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.; Authors - Qingzhou Chen ; KO Validated
human	IF	Nat Commun; Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites.; Authors - Marta Alenquer